Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

摘要

Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness,\udconformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with\udgood statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition\udspeed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in\udthroughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS\ud1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.